B-12
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Lyophilized Vitamin B₁₂ (Methylcobalamin) Powder
Key Features
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≥ 99 % Purity (HPLC‑Verified): Ensures minimal impurities for consistent, reproducible results.
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Freeze‑Dried Stability: Extended shelf life and easy transport; simply reconstitute in aqueous buffer when needed.
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Versatile Cofactor: Supports methionine synthase and methylmalonyl‑CoA mutase reactions critical to nucleotide synthesis and mitochondrial function.
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Low Endotoxin: Suited for sensitive in vitro assays without triggering unwanted immune responses.
Applications
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Cell Culture Supplement: Prevents B‑12 deficiency artifacts in hematopoietic, neuronal, and stem cell lines.
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Enzymatic & ELISA Standards: Ideal as a calibration standard in competitive binding assays and mass‑spectrometry workflows.
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Synthetic Biology & Biochemistry: Integrates seamlessly into microfluidic reactors and protocol development requiring cobalamin‑dependent enzymes.
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Pharmacokinetic Modeling: Accurate cofactor dosing for ADME/Tox studies and metabolic pathway investigations.
Specifications
Attribute | Detail |
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Form | Orange-Red to Red lyophilized powder |
Purity | ≥ 99 % (HPLC) |
Solubility | ≥ 50 mg/mL in water at 25 °C |
Storage | 2 – 8 °C, dry, protected from light |
CAS Number | 68‑19‑9 |

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